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Publications


  1. Nyakatura, E. K.; Soare, A. Y.; Lai, J. R.* Bispecific Antibodies for Vial Immunotherapy. Hum. Vaccin. Immunother. In press. [[Journal link]]
  2. Wec, A. Z.+; Nyakatura, E. K.+; Herbert, A. S.+; Howell, K. A.; Holtsberg, F. W.; Bakken, R. R.; Mittler, E.; Christin, J. R.; Shulenin, S.; Jangra, R. K.; Bharrhan, S.; Kuehne, A. I.; Bornholdt, Z. A.; Flyak, A. I.; Saphire, E. O.; Crowe, J. E.*; Aman, M. J.*; Dye, J. M*; Lai, J. R.*; Chandran, K.* A "Trojan Horse" Bispecific Antibody Strategy for Broad Proteciton against Ebolaviruses. Science, 2016, 354, 350-354.(+Equal contributors) [[Journal link]]
  3. Frei, J. C.; Lai, J. R.* Protein and Antibody Engineering by Phage Display. Methods Enxymol., 2016, 580, 45-87. [[Journal link]]
  4. Howell, K. A.; Qiu, X.; Brannan, J. M.; Bryan, C.; Davidson, E.; Holtsberg, F. W.; Wec, A. Z.; Shulenin, S.; Biggins, J. E.; Douglas, R.; Enterlein, S. G.; Turner, H. L.; Pallesen, J.; Murin, C. D.; He, S.; Vu, H.; Herbert, A. S.; Fusco, M. L.; Nyakatura, E. K.; Lai, J. R.; Keck, Z. Y.; Foung, S. K.; Saphire, E. O.; Zeitlin, L.; Ward, A. B.; Chandran, K.; Doranz, B. J.; Kobinger, G. P.; Dye, J. M.; Aman, M. J.* Antibody Treatment of Ebola and Sudan Virus Infection via a Uniquely Exposed Epitope within the Glycoprotein Receptor-Binding Site. Cell Rep., 2016, 15, 1514-1526. [[Journal link]]
  5. Frei, J. C.+; Nyakatura, E. K.+; Zak, S. E.+; Bakken, R. R.; Chandran, K.; Dye, J. M.*; Lai, J. R.*  Bispecific Antibody Affords Complete Post-Exposure Protection of Mice from Both Ebola (Zaire) and Sudan Viruses.  Sci. Rep., 2016, 6, 19193.  [[Journal link]]
  6. Uchime, O.+; Dai, Z.+; Biris, N.; Lee, D., Sidhu, S. S., Li, S., Lai, J. R.*, Gavathiotis, E.*  Synthetic Antibodies Inhibit Bcl-2-associated X Protein (BAX) through Blockade of the N-terminal Activation Site.  J. Biol. Chem., 2016, 291, 89-102.  [[Journal link]]
  7. Holtsberg FW, Shulenin S, Vu H, Howell KA, Patel SJ, Gunn B, Karim M, Lai JR, Frei JC, Nyakatura EK, Zeitlin L, Douglas R, Fusco ML, Froude JW, Saphire EO, Herbert AS, Wirchnianski AS, Lear-Rooney CM, Alter G, Dye JM, Glass PJ, Warfield KL, Aman MJ. Pan-ebolavirus and Pan-filovirus Mouse Monoclonal Antibodies: Protection against Ebola and Sudan Viruses.  J. Virol., 2015, 90, 266-289.  [[Journal link]]
  8. Frei, J. C.; Kielian, M., Lai, J. R.* Comprehensive Mapping of Functional Epitopes on Dengue Virus Glycoprotein E DIII for Binding to Broadly Neutralizing Antibodies 4E11 and 4E5A by Phage Display.  Virology, 2015,485, 371-382.  [[Journal link]]
  9. Malashkevich, V. N.; Higgins, C. D.+; Almo, S. C.*; Lai, J. R.*  A Switch from Parallel to Antiparallel Strand Orientation in a Coiled-Coil X-Ray Structure via Two Hydrophobic Mutations.  Biopolymers: Peptide Science, 2015, 104, 178-185. [[Journal link]]
  10. Liu, N.; Tao, Y.; Brenowitz, M. D.; Girvin, M. E.; Lai, J. R.* Structural and Functional Studies on the Marburg Virus GP2 Fusion Loop.  In press at J. Infect. Dis. [[Journal link]]
  11. Dai, Z.; Tao, Y.; Brenowitz, M. D.; Girvin, M.E.; Lai, J. R.*  Conditional Trimerization and Lytic Activity of HIV-1 gp41 Variants Containing the Membrane-Associated Segments.  Biochemistry, 2015, 54, 1589-1599.  [[Journal link]]
  12. Nyakatura, E. K.; Frei, J. C.; Lai, J. R.* Chemical and Structural Aspects of Ebola Virus Entry Inhbitors.  ACS Infect. Dis., 2015, 1, 42-52. [[Journal link]]
  13. Chen, G.+; Koellhoffer, J. F.+; Zak, S. E.+; Frei, J. C.; Liu, N.; Long, H.; Yi, W.; Nagar, K.; Pan, G.; Chandran, K.; Dye, J. M.; Sidhu, S. S.; Lai, J. R.* Synthetic Antibodies with a Human Framework that Protect Mice from Lethal Sudan Ebolavirus Challenge.  ACS Chem. Biol., 2014, 9, 2263-2273. [[Journal link]] Press coverage of this work: NY1, NY Daily News, FrontPageAfrica, Chemical & Engineering News, Quartz, Infection Control Today, Einstein1, Einstein2.
  14. Higgins, C. D.; Malashkevich, V. N.; Almo, S. C.; Lai. J. R.* Influcence of a Heptad Repeat Stutter on the pH-Dependent Conformational Behavior of the Central Coiled-Coil from Influenza Hemagglutinin HA2.  Proteins, 2014, 82, 2220-2229. [[Journal link]]
  15. Koellhoffer, J. F.+; Dai, Z+; Malashkevich, V. N.; Stenglein, M. D.; Liu, Y.; Toro, R.; Harrison, J. S.; Chandran, K.; DeRisi, J. L.; Almo, S. C.; Lai, J. R.*  Structural Characterization of the Glycoprotein GP2 Core Domain from the CAS Virus, a Novel Arenavirus-like Species.  J. Mol. Biol., 2014, 426, 1452-1468.   [[Journal link]]
  16. Koellhoffer, J. F.; Higgins, C. D.; Lai, J. R.*  Protein Engineering Strategies for the Development of Viral Vaccines and Immunotherapeutics.  FEBS Lett. 2014, 588, 298-307.  [[Journal link]]
  17. Liu, Y.; Higgins, C. D.; Overstreet, C. M.; Rai, K. R.; Chiorazzi, N.*; Lai, J. R.*  Peptides that Bind Specifically to an Antibody from a Chronic Lymphocytic Leukemia Clone Expressing Unmutated Immunoglobulin Variable Region Genes.  Mol. Medicine, 2013, 19, 245-252.  [[Journal link]]
  18. Higgins, C. D.; Koellhoffer, J. F.; Chandran, K.; Lai, J. R.* C-Peptide Inhibitors of Ebola virus Glycoprotein-Mediated Cell Entry: Effects of Conjugation to Cholesterol and Side Chain-Side Chain Crosslinking.  Bioorg. Med. Chem. Lett, 2013, 23, 5356-5360.  [[Journal link]]
  19. Harrison, J. S.*; Higgins, C. D.; O'Meara, M. J.; Koellhoffer, J. F.; Kuhlman, B. A.; Lai, J. R.*  Role of Electrostatic Repulsion in Controlling pH-Dependent Conformational Changes of Viral Fusion Proteins.  Structure, 2013, 21, 1085-1096.  [[Journal link]]
  20. Regula, L. K.; Harris, R.; Wang, F.; Higgins, C. D.; Koellhoffer, J. K.; Zhao, Y.; Chandran, K.; Gao, J.; Girvin, M. E.; Lai, J. R.* Conformational Properties of Peptides Corresponding to the Ebolavirus GP2 Membrane-Proximal External Region in the Presence of Micelle-Forming Surfactants and Lipids.  Biochemistry, 2013, 52, 3393-3404.  [[Journal link]]
  21. Stewart, A.; Harrison, J. S.; Regula, L. K.; Lai, J. R.*  Side Chain Requirements for Affinity and Specificity in D5, an HIV-1 Antibody Derived from the VH1-69 Germline Segment.  BMC Biochemistry, 2013, 4, 9.  [[Journal link]]
  22. Koellhoffer, J. L.+; Chen, G.+; Sandesara, R. G.+; Bale, S.; Saphire, E. O.; Chandran, K.*; Sidhu, S. S.*; Lai, J. R.*  Two Synthetic Antibodies that Recognize and Neutralize Distinct Proteolytic Forms of the Ebola Virus Glycoprotein.  ChemBioChem, 2012, 13, 2549-2557.  [[Journal link]]
  23. Koellhoffer, J. K.; Malashkevich, V. N.; Harrison, J. S.; Toro, R.; Bhosle, R. C.; Chandran, K.; Almo, S. C.; Lai, J. R.*  Crystal Structure of the Marburg Virus GP2 Core Domain in its Post-Fusion Conformation.  Biochemistry, 2012, 51, 7665-7775.  [[Journal link]]
  24. Harrison, J. S.; Koellhoffer, J. K.; Chandran, K.; Lai, J. R.*  Marburg Virus Glycoprotein GP2: pH-Dependent Stability of the Ectodomain alpha-Helical Bundle.  Biochemistry, 2012, 51, 2515-2525. [[Journal link]]
  25. Stewart, A.; Liu, Y.; Lai, J. R.*  A Strategy for Phage Display Selection of Functional Domain-Exchanged Immunoglobulin Scaffolds with High Affinity for Glycan Targets.  J. Immunol. Methods., 2012, 376, 150-155. [[Journal link]]
  26. Liu, Y.+; Regula, L. K.+; Stewart, A.; Lai, J. R.*  Synthetic Fab Fragments that Bind the HIV-1 Heptad Repeat Regions.  Biochem. Biophys. Res. Commun., 2011, 413, 611-615. [[Journal link]]
  27. Harrison, J. S.+; Higgins, C. D.+; Chandran, K.; Lai, J. R.*  Designed Protein Mimics of the Ebola Virus Glycoprotein GP2 alpha-Helical Bundle: Stability and pH Effects.  Protein Sci., 2011, 20, 1587-1596. [[Journal link]]
  28. Miller, E. H.+; Harrison, J. S.+; Radoshitzky, S. R.; Higgins, C. D.; Chi, X.; Dong, L.; Kuhn, J. H.; Bavari, S. ; Lai, J. R.*; Chandran, K. C.*  Inhibition of Ebola Virus Entry by a C-Peptide Targeted to Endosomes.  J. Biol. Chem. 2011, 286, 15854-15861.  [[Journal link]]
  29. Da Silva, G. F.; Harrison, J. S.; Lai, J. R.*  Contribution of Light Chain Residues to High Affinity Binding in an HIV-1 Antibody Explored by Combinatorial Scanning Mutagenesis.  Biochemistry 2010, 49, 5464-5472.  [[Journal link]]

  30. == Prior to Albert Einstein College of Medicine ==
  31. Pomerantz, W. C.; Grygiel, T. L. R.; Lai, J. R.; Gellman, S. H.  Distinctive Circular Dichroism Signature for 14-Helix-Bundle Formation by beta-Peptides.  Org. Lett. 2008, 10, 1799–1802.  [[Journal link]]
  32. Fischbach, M. A.; Lai, J. R.; Roche, E. D.; Walsh, C. T.; Liu, D. R.  Directed Evolution Can Rapidly Improve the Activity of Chimeric Assembly-Line Enzymes.  Proc. Natl. Acad. Sci. USA 2007, 104, 11951–11956.  [[Journal link]]
  33. Zhou, Z.; Lai, J. R.; Walsh, C. T.  Directed Evolution of Aryl Carrier Proteins: Determinants of Recognition in the Enterobactin Synthetase.  Proc. Natl. Acad. Sci. USA 2007, 104, 11621–11626.  [[Journal link]]
  34. Lai, J. R.; Koglin, A.; Walsh, C. T.  Carrier Protein Structure and Recognition in Polyketide and Nonribosomal Peptide Biosynthesis.  Biochemistry 2006, 45, 14869–14879.  [[Journal link]]
  35. Lai, J. R.; Fischbach, M. A.; Liu, D. R.; Walsh, C. T.  Localized Protein Interaction Surfaces on the EntB Carrier Protein Revealed by Combinatorial Mutagenesis and Selection.  J. Am. Chem. Soc. 2006, 128, 11002–11003.  [[Journal link]]
  36. Zhou, Z.; Lai, J. R.; Walsh, C. T.  Interdomain Communication Involving the Thiolation Domain of EntF Explored by Combinatorial Mutagenesis and Selection.  Chem. Biol. 2006, 13, 869–879.  [[Journal link]]
  37. Lai, J. R.; Fischbach, M. A.; Liu, D. R.; Walsh, C. T.  A Protein Interaction Surface in Nonribosomal Peptide Synthesis Mapped by Combinatorial Mutagenesis and Selection.  Proc. Natl. Acad. Sci. USA 2006, 103, 5314–5319.  [[Journal link]]
  38. Lai, J. R.+; Epand, R. F.+; Weisblum, B.; Epand, R. M.; Gellman, S. H.  Role of Salt and Conformation in the Biological and Physicochemical Behavior of Protegrin-1 and Designed Analogues: Correlation of Antimicrobial, Hemolytic and Lipid Bilayer-Perturbing Activities.  Biochemistry 2006, 45, 15718–15730.  [[Journal link]]
  39. Lai, J. R.; Fisk, J. D.; Weisblum, B.; Gellman, S. H.  Hydrophobic Core Repacking in a Coiled-Coil Dimer via Phage Display: Insights into Plasticity and Specificity at a Protein-Protein Interface.  J. Am. Chem. Soc. 2004, 126, 10514–10515.  [[Journal link]]
  40. Park, J. S.; Lee, H. S.; Lai, J. R.; Kim, B. M.; Gellman, S. H.  Accommodation of alpha-Substituted Residues in the alpha-Peptide 12-Helix: Expanding the Range of Substitution Patterns Available to a Foldamer Scaffold.  J. Am. Chem. Soc. 2003, 125, 8539–8545.  [[Journal link]]
  41. Raguse, T. L.; Lai, J. R.; Gellman, S. H.  Environment-Independent 14-Helix Formation in Short beta-Peptides: Striking a Balance between Shape Control and Functional Diversity.  J. Am. Chem. Soc. 2003, 125, 5592–5593.  [[Journal link]]
  42. Lai, J. R.; Gellman, S. H.  Reinvestigation of the Proposed Folding and Self-Association of the Neuropeptide Head Activator.  Protein Sci. 2003, 12, 560–566.  [[Journal link]]
  43. Raguse, T. L.; Lai, J. R.; Gellman, S. H.  Evidence that the beta-Peptide 14-Helix is Stabilized by beta-3-Residues with Side-Chain Branching Adjacent to the beta-Carbon.  Helv. Chim. Acta 2002, 85, 4154–4164.  [[Journal link]]
  44. Lai, J. R.; Huck, B. R.; Weisblum, B.; Gellman, S. H.  Design of Non-Cysteine-Containing Antimicrobial beta-Hairpins: Structure-Activity Relationship Studies with Linear Protegrin-1 Analogues.  Biochemistry 2002, 41, 12835–12842.  [[Journal link]]
  45. Raguse, T. L.; Lai, J. R.; LePlae, P. R.; Gellman, S. H.  Toward beta-Peptide Tertiary Structure: Self-Association of an Amphiphilic 14-Helix in Aqueous Solution.  Org. Lett. 2001, 3, 3963–3966.  [[Journal link]]
  46. Woll, M. G.; Lai, J. R.; Guzei, I. A.; Taylor, S. J. C.; Smith, M. E. B.; Gellman, S. H.  Parallel Sheet Secondary Structure in gamma-Peptides.  J. Am. Chem. Soc. 2001, 123, 11077–11078.  [[Journal link]]

 

Last Updated on Tuesday, 07 February 2017 09:38